![]() Of 4,951 porcine SCNT-TG embryos generated, 850 were cultured in the presence of 1 µg/ml doxycycline in vitro. The Tet-on system was then tested in the generated porcine SCNT-TG embryos. Somatic cell nuclear transfer (SCNT) was then performed using these donor cells. At 1 day after treatment with 1 µg/ml doxycycline, the fluorescence intensity of these cells was increased. ![]() Porcine fetal fibroblasts were infected with a universal doxycycline-inducible vector containing the target gene enhanced green fluorescent protein (eGFP). The Tet-on system was first tested in infected donor cells. The aim of this study was to produce pigs expressing a tetracycline (Tet)-inducible transgene. Transgenic (TG) pigs are widely used as models of human diseases. Please note our licence (freeware) for this product before you use the software.Pigs are anatomically and physiologically closer to humans than other laboratory animals. Pay as much as you want! This software can be downloaded for free and you can use it without any restrictions for non-commercial purposes. Windows, Linux and macOS Linux, Windows and macOS macOS, Windows and Linux More languages.ġ.75 MB to 3.64 MB - depending on the Program Version ![]() English, Dansk (Danish), Deutsch (German), Español (Spanish), Français (French), Italiano (Italian), Nederlands (Dutch), Polski (Polish), Português do Brasil (Brazilian Portuguese), Русский (Russian), العربية (Arabic), 日本語 (Japanese) and 简体中文 (Simplified Chinese).
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